Plasmid

Part:BBa_J119141:Design

Designed by: Todd Eckdahl, Claire Shinneman   Group: Eckdahl Lab   (2013-04-15)


Device for Testing New Promoters via BsgI Golden Gate Assembly


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1589
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 772
    Illegal NheI site found at 795
    Illegal NheI site found at 1624
    Illegal NheI site found at 1647
    Illegal SpeI site found at 1590
    Illegal PstI site found at 1604
    Illegal NotI site found at 1597
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1590
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1590
    Illegal PstI site found at 1604
    Illegal AgeI site found at 1462
    Illegal AgeI site found at 1574
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 72


Design Notes

J119137 (Plasmid) + J23100 (Promoter)


Source

Groningen 2009, Warsaw 2010, Claire Shinneman, Paul Frederick Group: iGEM2006_Davidson

References